Mouse anti-V5 tag mAb, clone 7-1-8
Mouse anti-V5 tag mAb is specific to GKPIPNPLLGLDST (V5) tag which can be
applied to several immunoassays including ELISA, WB, IP, IFA and FACS. This
antibody has been identified in several sources (bacteria, yeast and mammalian
cells) of V5 tag fusion protein.
applied to several immunoassays including ELISA, WB, IP, IFA and FACS. This
antibody has been identified in several sources (bacteria, yeast and mammalian
cells) of V5 tag fusion protein.
Product type:
Monoclonal
Concentration:
1 mg/mL
Immunogen:
Pepetide: GKPIPNPLLGLDST (V5)
Host:
Mouse
Reactivity:
Recognizes tag sequence of GKPIPNPLLGLDST on either the N- or C-terminus of recombinant fusion protein.
Isotype:
IgG2b kappa
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 0.01% thimerosal, pH 7.4.
Storage:
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Application:
ELISA, WB, IP, IFA, FACS
Manual:
Note: Application concentration may be various determined by the end user.
Shipping Conditions:
Blue ice
Monoclonal
Concentration:
1 mg/mL
Immunogen:
Pepetide: GKPIPNPLLGLDST (V5)
Host:
Mouse
Reactivity:
Recognizes tag sequence of GKPIPNPLLGLDST on either the N- or C-terminus of recombinant fusion protein.
Isotype:
IgG2b kappa
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 0.01% thimerosal, pH 7.4.
Storage:
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Application:
ELISA, WB, IP, IFA, FACS
Manual:
| Application | Dilution factor | Final concentration |
| ELISA | 1:5000-20000 | 0.05-0.2 μg/mL |
| WB | 1:1000-10000 | 0.1-1 μg/mL |
| IP | 1:500-2000 | 0.5-2 μg/mL |
| IFA | 1:500-1000 | 1-2 μg/mL |
| FACS | 1:500-1000 | 1-2 μg/mL |
Shipping Conditions:
Blue ice
